Vorträge

Modeling and simulation of diffusion-based transport processes

in cell nuclei, in vivo

Dr. Dietmar Volz
Abt. Intelligent Bioinformatics Systems
Deutsches Krebsforschungszentrum Heidelberg

Abstract:

The most recent modeling approaches to investigate the principles governing nuclear architecture [Phair and Misteli, High mobility of proteins in the mammalian cell nucleus, Nature v404, p604-9, 2000] deal with diffusion-based transport processes of flourescent molecules inside the nucleus. Hereby, the "well-mixed" compartmental assumption fits the quantitative dynamics data, but contradicts long held models of nuclear organization.

In order to test this assumption, a more accurate diffusion model including the nucleus boundary has been taken and compared to the fundamental solution of a time-dependent diffusion equation. For the simulation, an advanced PDE solution tool called 'UG' has been used and applied to bleaching data of high resolution in order to estimate an appropriate diffusion tensor.

Zeit: Donnerstag, den 27. Juni 2002, 16 Uhr c.t.

Ort: Institut für Neuro- und Bioinformatik
        TZL, Seelandstr. 1a, Geb. 5
        Seminarraum OG.3